Part:BBa_K4361114

Blc operator sequence SDM deletion

This part shows the blc operator sequence after site-directed mutagenesis of the (Part:BBa_K4361111) with primers Part:BBa_K4361112 and Part:BBa_K4361113, resulting in the deletion of the adenine in position 20.

It is known from literature that this mutation disrupts the binding between BlcR and the blc operator sequence [1]. This way BlcR cannot inhibit downstream gene expression.

The mutated blc operator sequence is incorperated in a superfolded GFP production plasmid, see Part:BBa_K4361115.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1] Pan, Y., Fiscus, V., Meng, W., Zheng, Z., Zhang, L.-H., Fuqua, C. and Chen, L. (2011). The Agrobacterium tumefaciens Transcription Factor BlcR Is Regulated via Oligomerization. The Journal of Biological Chemistry, [online] 286(23), pp.20431–20440. doi:10.1074/jbc.M110.196154